Sentence examples for activity was measured using the fluorogenic from inspiring English sources
For measurement of apoptosis induction, caspase-3 like protease (DEVDase) activity was measured using the fluorogenic substrate acetyl-ℒ-aspartyl-ℒ-glutamyl-ℒ-valyl-ℒ-aspartic acid-7-amino-4-methylcoumarin (DEVD-AMC, Peptide Institute, Osaka, Japan), as described previously.
Caspase-3 activity was measured using the fluorogenic substrate Ac-DEVD-AMC (Bachem, Weil am Rhein, Germany).
Milk NAGase activity was measured using the fluorogenic method of Kitchen and co-workers (1978) [ 30] with a microplate modification developed by Mattila [ 31].
24 PE activity was measured using the fluorogenic substrate, Z-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC; Bachem, Budendorf, Germany), as described previously.
Briefly, enzyme activity was measured using the fluorogenic substrate Mca-PLGL-Dpa-AR (Calbiochem) and a Tecan M1000 plate reader at 5 nM enzyme and 5 μM substrate in 50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM CaCl2, 0.05% Brij-35.
Heart and lung ACE2 activity were measured using the fluorogenic substrate Mca-Ala-Pro-Lys(Dnp -OH (Anaspec, CA, USA).
GALC activity was measured using the artificial fluorogenic substrate 4-methylumbelliferone-β-galactopyranoside.
Renal ACE2 activity was measured using the caspase-1 fluorogenic substrate 7-Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp -OH (R&Dnp -OHms, MN, USA), wheR&DMca iSystemsthoxycouMNrin-4-yl)-acetyl and Dnp is 2,4-dinitrophenyl.
Protein pellets were resuspended in 100 mM Tris-Cl (pH 7.4 /150 nM NaCl and activity was measured using the aforementioned assay conditions and panel of fluorogenic substrates.
The fluorogenic peptide Ac-DEVD-AMC was from Bachem (Voisins les Bretonneux, France) and the lactate dehydrogenase (LDH) activity was measured using the Cytotox 96® assay from Promega (Charbonnières, France) as described previously [ 17, 18, 21].
Cathepsin-L activity was measured using the synthetic substrate Z-Phe-Arg-AMC essentially according to [ 4, 5] as described in the manufacturer's protocol (InnoZyme™ Cathepsin-L Fluorogenic Activity Kit, Calbiochem, San Diego, CA).
