The phrase "activity was determined using the fluorogenic" is correct and usable in written English.
It can be used in scientific or technical contexts when describing how a specific activity or measurement was assessed through a fluorogenic method.
Example: "The enzyme activity was determined using the fluorogenic substrate, allowing for precise quantification of the reaction rate."
Alternatives: "activity was assessed using the fluorogenic" or "activity was measured using the fluorogenic".
Chymotrypsin activity was determined using the fluorogenic substrate Suc-Leu-Leu-Val-Tyr-7-amido-methylcoumarin (suc-LLVY-AMC, 50 µM to in vitro experiments and 25 mM to cell extracts, BioMol) which was added to 200 ng of 20S proteasomes (BioMol, in 20 mM Tris, pH 7.2, 1 mM EDTA, 1 mM DTT) or to 10 µg of cell extract (in 20 mM Tris-HCl, pH 7.5).
Serum TRAP isoenzyme 5b (TRAP 5b) activity was determined using the fluorogenic substrate, naphthol AS-BI phosphate (Sigma-Aldrich).
The activity of MMP12 was determined using the fluorogenic substrate Mca-PLGLDL Dpa AR (Mca-PLGLDL Dpa ARuMcain-4-yl)acetyl, Dpa, N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Anaspec).
The plasma concentration of EPA in humans consuming large doses would be in the range 100 200 μ M. To determine if proteasome proteolytic activity was also increased by 15(S -HETE, the 'chymotrypS -HETEe' enzyme actheity, the predominant proteolytic activity of the proteasome, was determined using the fluorogenic substrate Suc LLVY-MCA.
The β-galactosidase activity of mutant pools and clones was determined using the fluorogenic substrate fluorescein-di-β-D-galactopyranoside (FDG, MarkerGene Technologies Inc ,Eugene, OR, USA), which allows for an accurate determination of the LacZ activity over at least 4.5 orders of magnitude.
Steady-state kinetics of wild-type hOGA and CpOGA mutants were determined using the fluorogenic substrate 4MU-NAG (4-methylumbelliferyl N-acetyl-β D-glucosaminide; Sigma).
To ascertain the effect of TCTP on caspase activity, caspase-specific inhibitors were added into the reactions and caspase activity was determined using a fluorogenic substrate that emits fluorescence when caspases cleave it.
MMP7 activity was determined using a fluorogenic MMP7 substrate assay (Merck Millipore, Nottingham, UK) and compared to negative control (chondrogenic medium) and positive control (recombinant human MMP7) according to the manufacturers' instructions.
Total β-galactosidase activity was determined using 4-methylumbelliferyl-β-D-galactopyranoside as the synthetic fluorogenic substrate, specific for β-galactosidase as previously described [9].
Intracellular ROS level was determined using the oxidant-sensitive fluorogenic probe CM-H2DCFDA (5- and-6 -chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester) from Invitrogen, Carlsbad, California.
