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The specificity of this activity was confirmed using the CDK5 inhibitor roscovitine.
mPAP was heat-inactivated by incubating at 65°C for 40 min. Loss of activity was confirmed using the EnzChek Phosphatase Assay Kit (Invitrogen, E12020) following the manufacturer's protocol.
Dose-dependent anti-DENV activity was confirmed using a dengue reporter virus.
The anti-DENV activity was confirmed using a Renilla luciferase expressing DENV reporter.
The lack of kinase activity was confirmed using human recombinant proteins [ 36, 68].
This low level of GUS activity was confirmed using a GUS quantitative assay.
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The effects of biotin on CYP1A activity were confirmed using several different strategies.
The storage conditions that promoted the stability of LLO haemolytic activity and cytotoxic activity were confirmed using sheep erythrocytes and Jurkat cells, respectively.
The superior accuracy of activity measures was confirmed using Random Forest and predictive modeling techniques.
The ribonuclease activity of GCN4 LZ35 was confirmed using commercial RNA (baker's yeast; Sigma) as substrate [Fig. S5].
Later, the presence of protease inhibitory activity of seed was confirmed using ammonium sulphate precipitated fractions and elutes of SEC.
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