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Removal of TSPs from astrocyte-conditional medium diminishes the synaptogenic activity of the medium.
NaCl is often used for the simulation of osmotic stress and is also known to reduce the water activity of the medium inducing water stress (Kets et al. 1996; Heipieper et al. 1996; Halverson and Firestone 2000).
As high salt concentration tends to greatly reduce the water activity of the medium it is reasonable to apply this kind of enzymes in solvent tolerant process [9].
It is interesting to note that the killing activity of the medium is lower than the killing activity of the cells (compare Figures 1A and 4A).
Such assumption is no longer valid in in vivo experiments in actual neural systems, where noise is the result of the inherent activity of the medium (which could be, for instance, the highly irregular spontaneous activity of other cortical regions projecting to the structure of interest) and, therefore, not easily controlled by the experimentalist.
The luciferase activity of the medium and the cells were measured separately.
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In case of collision of RTS packets, activity on the medium has the PGF of Ct z) = zrts+cts+sifs.
The LDH activity of the incubation medium, cell culture medium and cell lysates was determined and expressed as percentage of the total activity for each condition.
Raising the pH of the liquid medium up to 8.2 did not result in detectable cell lysis, as determined by microscopic examination, viability counting or release of alkaline phosphatase activity to the medium.
Angiogenic activity of the coculture medium was compared with the culture medium from both MDA-MB-231 and Mφ cultured individually in the chick chorioallantoic membrane (CAM) model.
Daily lactate dehydrogenase activity measurements of the medium and immunofluorescence end-point staining proved the viability of the tissues and the maintenance of differentiated cellular phenotypes.
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