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Nevertheless, it remains unclear whether such an activity is obligatory for all or any of Fz receptor signaling functions.
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None of the activities were obligatory.
Creatine kinase-deficient mice survive for a long time with only mild dysfunction, so obviously CK activity is not obligatory for cardiac function, but the finding that male M/Mt-CK−/−(129/Bl6) mice eventually develop heart failure suggests that loss of CK places a chronic strain on the heart and that gender modulates the effects of CK depletion.
Activity of methanogenic Archaea is obligatory for methane production and can be addressed by physiological parameters such as (i) the methane productivity of a given fermenter sludge or (ii) transcription of the key enzyme of methanogenesis, and relating these to the concentration of methanogens in a given sludge volume.
The demonstration of differential activation of prefrontal subregions in the intact brain during different demands on decision-making and choice behavior does not imply that activity in these different regions is obligatory for these functions to occur normally.
It is generally accepted that engagement with social networks is obligatory for the success of an e-business activity.
There is obligatory scatology.
Half board is obligatory.
Indeed, it is obligatory.
Expertise of international standards is obligatory.
The "by all accounts" is obligatory.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com