Due to the limited availability and the high cost to produce large-scale 225Ac/213Bi generators, preclinical studies with 213Bi are often being performed using relatively low activity generators (e.g. 222 MBq 225Ac).
Under these conditions, the standard clinical labelling procedure for 213Bi, which was designed for high activity generators (up to 4 GBq) with a reaction volume of 2 mL at > 0.7 Osmol.L−1 (Kratochwil et al. 2014), probably needs to be adjusted.
Due to the uncertainty in the localisation of epileptic activity generators, icEEG electrodes are frequently placed over regions that turn out to be apparently free of pathology thereby providing an opportunity to study normal brain activity [Conner et al., 2011; Hermes et al., 2012; Khursheed et al., 2011].
fMRI held promise to circumvent the limitations imposed by the EEG inverse problem, which is particularly challenging for deep and extensive epileptic activity generators [Chaudhary et al., 2012a; Jacobs et al., 2008b; Salek-Haddadi et al., 2006; Thornton et al., 2010a, 2011; Zijlmans et al., 2007].
To date, the required Ac to build a high-activity generator system to provide sufficient Bi for clinical use has only been possible by combining the maximum activity currently available from the three producers, i.e. Oak Ridge National Laboratory (Oak Ridge, TN), the Institute for Transuranium Elements (Karlsruhe, Germany) and the Institute for Physics and Power Engineering (Obninsk, Russia).
The most practical method to achieve high SA radiolabelling for preclinical applications is the use of a higher activity 225Ac/213Bi generator to accomplish the requirements needed for preclinical applications.
Thus, based on the results from this study, it appears that the MeA and CeA per se are not involved in the normal regulation of LH pulses under nonstressed conditions, but nonetheless their activation under stressful conditions consequently inhibits GnRH pulse generator activity.
Elution yield was calculated as percentage of nominal 68Ge activity in the generator.
Since activity yield from generator, yield of synthesis as well as delay between synthesis and cell culture experiments differed between the experimental series, peptide amount kept constant between the separate series.
They are intended to elaborate the measurements acquired from the field, by means of distributed measurement systems, along with all the a priori information that can be collected about load and generator activity.
