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Activity assignments based on triplicate runs.
The triplicate data also provided us with the opportunity to evaluate the rationale for the activity assignments based on curve class.
The exact duration of compound treatment prior to application of the MMP detection dye will likely have an effect on the activity assignments mentioned above.
We used the results of the triplicate runs for each channel [red and green fluorescence, ratio (red/green), cytotoxicity, and compound autofluorescence] to make compound activity calls (see "Activity assignments based on triplicate runs"; see also Supplemental Material, "Reproducibility call," Tables S2, S3).
Decisions on compound activity took into account triplicate runs of not only the ratio (red/green) readout in the MMP assay but also each fluorescence channel individually and the cytotoxicity results (see "Activity assignments based on triplicate runs"; see also Supplemental Material, "Reproducibility call," Tables S2, S3).
Similar(55)
Activity assignment to individual catheter positions might not be feasible.
The final data collection including the thresholds for activity assignment can be reviewed in the supplement [see Additional file 2].
One point of discussion is the threshold setting for activity assignment, in that it followed no algorithmic procedure.
This paper proposes modified particle swarm optimization to solve the problem of activity assignment of fourth party logistics (4PL) with preemptive structure.
Although our threshold settings may have enhanced the aforementioned difference in activity assignment, these were more likely due to changes in the experimental setup, or variance in the Drosophila stock between the measurements of the training and test sets.
Compound-level activity assignment is described below.
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