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The behavioral activities were assessed using actophotometer, novel object recognition test (ORT), Morris water maze (MWM) followed by biochemical assessments, determination of proinflammatory cytokines and mediators (TNF-α, IL-1β and PGE2), β-A1 42 levels and histopathological analysis.
Caspase activities were assessed using Ac-DEVD-AMC or Ac-IETD-AMC substrates (Bachem) as described elsewhere [34].
Symptoms and activities were assessed using the WOMAC.
GST activities were assessed using an assay kit from Cayman and following the manufacturer's protocol.
Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay.
APF and APM activities were assessed using the sulphorhodamine B and lactate dehydrogenase assays, respectively.
Similar(36)
Consequences of mGluR5 inactivation for hippocampal network activity were assessed using analysis of intrahippocampal theta and gamma oscillations.
Firefly and Renilla luciferase activity were assessed using the Luciferase® Reporter Assay (Promega) following the manufacturer's instructions.
The level of physical activity were assessed using the International Physical Activity Questionnaire (IPAQ) [ 26].
Pain and disease activity were assessed using an EQ-5D (EuroQoL-5 Dimensions) visual analogue scale.
The dietary habits and physical activity were assessed using a questionnaire administered by an interviewer.
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