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ALT and AST activities were analyzed using commercial kits from Nanjing Jiancheng Bioengineering Institute.
(A) The cell-free caspase-3, -8, and -9 activities were analyzed using specific substrates.
Luciferase activities were analyzed using the Dual-Luciferase Kit (Promega), with firefly fluorescence units normalized to renilla luciferase fluorescence units (BMG microplate reader).
Fe3+-reducing activities were analyzed using the ferrozine assay (30).
Luciferase activities were analyzed using a luminometer (Lumat LB.9507, EG and G, Berthold, Germany).
Forty-eight hours after transfection, the luciferase activities were analyzed using the Dual Luciferase system.
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Differences between effects of vehicle and URB597 or URB694 on lipid levels and FAAH activity were analyzed using one-way ANOVA.
In addition, to address the possible mechanism of OA chondrocytes [Ca2+]i signaling alternation and downstream regulation of [Ca2+]i signaling, the protein level of T-type voltage-dependent calcium channels (VDCCs) and Nfatc2 activity were analyzed using total knee arthroplasty (TKA) samples.
Cyclotraxin-B reversibility and 30-min cumulative counts in locomotor activity were analyzed using Student's t test.
The temporal dynamics of the spectral components of cortical source activity were analyzed using a wavelet transform.
The effect of different doses of the A2AR antagonists on locomotor activity were analyzed using a one-way analysis of variance (ANOVA), followed by Newman-Keuls' post-hoc test.
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