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At 48 h hours after transfection, Renilla and Firefly luciferase activities were analysed using the Dual Luciferase Assay (Promega).
Caspase 3/7, 8, and 9 activities were analysed using the appropriate Caspase-Glo kit (Promega, Madison, WI, USA) for cell-based assays and following the manufacturer's instructions.
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The effects of disease and drug on behavioural performance and regional brain activity were analysed using general linear models.
Samples for anti-Xa activity were analysed using a validated chromogenic assay kit (COAMATIC™ Heparin, Chromogenix, Instrumentation Laboratory Company, Lexington, MA, USA).
Meat-cooking methods, HCAs, B(a P, and total mutagenic activity were analysed using Computerized Heterocylic Amines Resource for Research in Epidemiology of Disease (CHARRED) (Sinha et al, 2005).
PC activity was analysed using a haemostasis laboratory analyser (BCS® XP; Siemens).
Creatine kinase activity was analysed using a commercially available kit (Pointe Scientific, Inc., Canton USA) with modifications to the protocol to allow for running the samples at 25°C.
The NF κB activity was analysed using the Dual-Luciferase RePromegaAssay System (Promega).
Enzyme activity was analysed using Sigma Plot Enzyme Kinetics Module (Systat Software, Richmond, CA).
Luciferase activity was analysed using the Steady-Glo Luciferase assay kit (Promega).
Cells were then harvested and luciferase activity was analysed using a manufactured kit (Promega UK Ltd) following the recommended guidelines.
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