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Our initial protein sequencing studies 6 suggested that the enzyme activity was likely to be of human origin.
In the human system IRAK-1/IRAK-2 deficientficells cells are not available and we used the well-described capacity [ 1] of IRAK-M to drive NF-κB activation spontaneously when transfected in 293T cells as a model system to study the NF-κB activating activity of human IRAK-M in cells of human origin.
Due to its high conservation of core processes shared with higher eukaryotes, yeast can provide a relevant cellular environment for the characterization of exogenous pathways, for example, of human origin, when confounding activities are removed in yeast via evolution or genetic engineering.
So does recombinant mucus protease inhibitor (MPI), the only previously known airway protease inhibitor of human origin with anti-IAV activity (Beppu et al., 1997).
GLI2 of human origin demonstrated slightly higher transcriptional activity than mouse Gli2 in MDA-MB-231 cells.
Our studies in human leukocytes confirmed that the inhibitory activity of BRO on leukotriene release observed in the rat also applies to cells of human origin (Fig. 3).
All cDNAs were of human origin.
The cells were too small to be of human origin.
All constructs are of human origin.
All these cells were of human origin.
All enzymes used were of human origin, purified or recombinant.
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