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Fig. 3 Laccase activities of co-transformants.
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For example, the enzymatic activity of co-transformants EuHR-2 (4000 ± 90 rlu) and EuHR-12 (1,961,000 ± 132,000 rlu) differed by a factor of nearly 500.
Although the laccase activity of co-transformant L 61 is lower than that of typical white-rot fungus T. versicolor, the activity was similar to that of P. coccineus.
Although the laccase activity of co-transformant L 61 was lower than that of typical white-rot fungus T. versicolor, the activity was similar to that of P. coccineus (Fig. 3b).
Transformants with elevated laccase production might be generated by modifying the pGL promoter region and/or by screening among a larger number of co-transformants.
In contrast, no positive interaction was detected in a series of co-transformants used as negative controls.
As shown in Fig. 4A, a group of co-transformants grown on selective medium was successfully isolated (also see Figure S3).
In these in-gel-luciferase activity assays, co-transformants generated with the pHRLucP plasmid exhibited strong luciferase signals of the expected size (~21 kDa).
We assayed the ability of co-transformant L 61 to degrade lignin using wood meal derived from Japanese cedar.
The enzymatic activity of pHsp70A-GLuc co-transformants differed by a factor of about 70.
It should be noted that the luciferase activity levels in co-transformants were quite variable.
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