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Moreover, antioxidant activities of calli extract were assayed.
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Transposon display (TD) analysis was performed using combinations of MseI-adaptor-primers with two consecutive mPing-specific primers at the 5' end (named as TAILmp-1 and -2) to assess the transpositional activity of mPing in calli and regenerants of the three rice ssp.
Split hypocotyl segments and double amounts of KNO3 during induction of calli were beneficial to the emerge of embryogenic calli.
Proliferation of calli was achieved on transfer of the one month old calli to the same fresh medium this in line with the previous report.
Using the protocol developed, 34% of the calli infected were hyg B resistant, and the overall plant transformation frequency (number of independently transformed plants over number of calli infected) was 8%.
We harvested both sets of calli 24 h later.
dwf7-1 calli displayed only marginal signs of shoot induction; parts of calli turned green, but almost no shoots were produced.
On subsequent subcultures of calli on the same medium, shoot proliferation started from these calli, within 3 weeks (Fig. 1D).
Weight of calli transformed with HPT-GFP vectors were measured per dish before and after the first round of selection to determine the callus growth activities conferred by each promoter.
In vitro plant regeneration was studied and analyzed 15, 20 and 30 days after subculture of calli on either A4 medium (CA4 calli) or on A10 medium (CA10 calli).
With cacao tissue cultures, we and our collaborators have frequently observed two types of calli, those that produce abundant embryos (embryogenic calli) and those that produce few if any embryos (non-embryogenic calli) (unpublished observations).
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