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Cells were then plated on blood agar plates with antibiotics and incubated for up to 48 h. S. pneumoniae strain Ali 9.2 containing a kan-rpsL + cassette in place of the ali gene, was used as the positive control and a no-DNA control plate was included to check for background resistance activities in the recipient cells.
We also transplanted the labeled islets with the nestin (the marker of neurovascular stem/progenitor cells; Dore-Duffy et al., 2006, Mignone et al., 2004, Treutelaar et al., 2003) promoter-driven green fluorescence protein (GFP) expression in the Schwann cells and pericytes (Alliot et al., 1999, Clarke et al., 1994, Frisen et al., 1995) to trace their locations and activities in the recipient.
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However, the luciferase activity in the recipient cells was significantly reduced (Fig. 6D).
In contrast, IMP1 overexpression increased the luciferase activity in the recipient cells in an IMP1 dose-dependent manner (Fig. 1F).
In contrast, the normalized firefly luciferase activity in the recipient cells infected with MLV-luc produced in the presence of IMP1 was much higher than that in the presence of empty vector or IMP3 (Fig. 1C).
Consistent with the previous results (Fig. 1C), when the same samples of virus were used to infect 293 cells, the luciferase activity in the recipient cells infected with virus produced in IMP1-expressing cells was about 5-fold of that in the cells infected with virus produced in the control cells (Fig. 2F).
The "pull factors" are the green pastures and other activities that influence recruitment in the "recipient" western developed nations [ 3].
In terms of strategizing vaccination regimens to take advantage of both endogenous and passive immunity, the persistence of functional activity of maternal CMI in the recipient warrants elucidation.
At 1 year after surgery, 10 patients showed the same or slightly increased activity at the recipient site in comparison to the preoperative levels.
The delivery of protein drugs has often led to the rise in production of neutralizing antibodies which may diminish or abolish the activity of a peptide hormone in the recipient.
This might suggest that ComM activity contributes more to transformability in the recipient background, but the requirement for ComM was substantially stronger in the 86-028NP donor background (wild type: 3.9 ± 0.6 × 10−6 KanR/CFU; comM deletion: <7.9 ± 0.2 × 10−9).
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