Then, specific activity calculations need to individualize the PP present in the cross-linked aggregates.
However, the published literature evidences that a) much specific non-systematic exploratory work is being done and, b) recovered activity calculations in CLEAs still need to be optimized.
Zuber, G; Goldsmith, M-R; Beratan, DN; Wipf, P, Towards Raman optical activity calculations of large molecules., Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry, vol. 6 no. 4 (April , 2005, pp. 595-597, [doi].
To compensate for possible presence of cell debris etc., absorbance at 550 nm was included and compensated in the activity calculations, which when multiplied by 1.75 estimates the light-scatter at 420 nm (Stephenson 2016).
To be more confident with the modeling results, the calibration step was performed based on the salt-free experiment for which the ion activity calculations using the Debye Hückel approach are perfectly valid.
For specific activity calculations, the radioactivity of the injected product for the radiochemical analysis was measured with a Capintec R15C dose calibrator (Capintec, Inc., Ramsey, NJ, USA), while the mass of SIG343 and SIG353 was determined by comparing the area of the UV absorbance peak corresponding to the carrier product with a calibrated standard curve relating its mass to UV absorbance.
The activity calculations were based on Michaelis-Menten first-order kinetics.
Vela and Imig activities were determined on the day of injection and were used for pre- and post-injection activity calculations.
Intracellular enzyme measurements and activity calculations were performed as described previously (Battaglia et al. 2011).
The extinction coefficients used for activity calculations were ε340 = 6.22 mM–1 cm–1 for deamino-NADH and ε420 = 1.00 mM–1 cm–1 for K3Fe CN 6.
