Many transcriptional co-activators and co-repressor complexes with histone-modifying activities bind transcription factors in vivo, suggesting that targeted chromatin remodeling may be important for the function of transcription factors [13], [14].
Our observations that many proteins with enzymatic activities bind to RNAs and potentially participate in RNA regulation are reminiscent to previous observations stating RNA-binding functions for several mammalian enzymes (reviewed in [12], [13]).
The actual activity bound to the cells was also measured in an automatic gamma counter.
The radioactivity related to the bacteria was expressed in percentages of the added 99mTc activity bound to viable bacteria in regard to the total 99mTc activity.
From the separately determined total radioactivity of the blood sample, the average activity bound to a single microsphere was then calculated.
On the other hand, it is questionable to what extent the strategy-level alone can grasp societal impacts of innovations as future-oriented activity bound with uncertainties.
The TALE SIDs repressed luciferase reporter activity, bound their genomic target sites, and repressed AXIN2 and MYC expression in HEK293 cells.
The percentage of tracer uptake stated as percentage injected dose per gram of tissue (%ID/g) was calculated as the activity bound to tissue per organ weight per actual injected dose, decay-corrected to the start time of counting.
The amount of activity bound to the peptide was then determined using a γ-counter.
Proteins associated with RESCUE activity bound to the column and were eluted at low salt concentrations (Figure S2).
