Proteolytic activities were continuously monitored in 2 hr at 37°C by measuring the release of the fluorescent group, 7-amido-4-methylcoumarin (AMC), at a fluorescence plate reader (Spectramax M2, Molecular Devices, Sunnyvale, CA) with excitation and emission wavelengths of 380 and 460 nm, respectively.
Uterine activity was continuously monitored and quantified as the hourly contraction area (millimeters of mercury · seconds per hour) in the experimental group and a control group (n = 5) that received interleukin 1β alone.
ENaC activity was continuously monitored in paired cell-attached experiments.
Wheel-running activity was continuously monitored and recorded by a computer system (The Chronobiology Kit; Stanford Software Systems, CA).
Locomotor activity was continuously monitored throughout the whole experiment including 4 days of baseline phase and 20 days of stress phase.
One-year old wild-type and Myog-deleted mice were individually caged in a Comprehensive Laboratory Animal Monitoring System (Columbus Instruments) for a total of three days during which time food intake, O2 consumption and CO2 production (from which energy expenditure and RER was calculated), and activity were continuously monitored.
Aconitine (10 μg/mL) was continuously infused at a speed of 0.2 mL/min, and ECG activity was continuously monitored and timed.
Muscle activity was continuously monitored throughout steady state walking sessions and collected using EMGworks Acquisition software program (Delsys EMGworks 4.0, Delsys, Boston, MA).
Extracellular electrical activity was continuously monitored (signal was acquired from all 256 channels, 10 kHz) and spikes crossing a threshold set at 3 times the standard deviation of the noise on each channel were recorded and stored for off-line analysis.
These four regions are continuously monitored.
Fetuses are continuously monitored with fetal echocardiography.
