The phrase "active translation" is correct and usable in written English.
It can be used in contexts related to language translation, particularly when referring to the process of translating text or speech in real-time or dynamically.
Example: "The conference featured a session on active translation, allowing participants to engage in discussions in multiple languages simultaneously."
Alternatives: "real-time translation" or "dynamic translation".
Finally, we sought to determine whether the ribosomes that appear to translate extensions are engaged in active translation, as opposed to some aberrant process of stalling or slippage (e.g., as described in Skabkin et al., 2013).
We pair this data with ribosome profiling, the deep sequencing of ribosome-protected mRNA fragments during active translation; this approach offers significant insight into proteome dynamics and translational regulation not available by monitoring transcript expression alone (Ingolia et al., 2009, 2011; Stern-Ginossar et al., 2012).
The first possibility is that DAZL activates tdrd7 translation without affecting poly(A) tail length, and subsequently active translation results in the polyadenylation of tdrd7 mRNA.
Notably, prolonged disruption of the secondary structure and, hence, active translation of yidC2 are brought about by the stall of the mifM-translating ribosome at a few codons upstream of the stop.
Total RNA from RNA-Seq indicates RNA synthesis activity, while the ribosome protected fragments (RPF) serve as the indicator of active translation.
Active translation destabilizes mRNA structure in cells.
Fractionation of polysomes allows examination of mRNA bound to more than one ribosome and, therefore, contains mRNAs engaging in active translation.
Taken together, these results suggest that active translation impedes miRNA-programmed RISC association with target mRNAs and support a mechanistic explanation for the localization of most miRNA target sites in noncoding regions of mRNAs in mammals.
There may thus be functional overlap between RAN translation and the translational mechanism used by single-stranded alphaviruses, where a hairpin structure within the coding region just 3′ to the start codon maintains active translation in the setting of eIF2α phosphorylation58.
An analysis of sorted CCR2− macrophages from nicotine-treated mice confirmed significantly higher levels of active translation for the M2-related genes IL-10 and Arg1 than were found in sorted macrophages from normal, injured, or locally ISO-treated corneas (Fig. 7e, bottom; factorial-design ANOVA, P < 0.01).
Cells in the log phase (A580 = 0.4 0.6) had an active translation system and protein biosynthesis was efficient.
