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Scatterplot of the fraction of active reactions in monocyte derived macrophages versus the fraction of active reactions in monocytes.
EFMs are minimal in the sense that the active reactions in an EFM cannot be a subset of the active reactions in another EFM.
After identifying active reactions in defined model simulations (described below), we examined whether there was a strong association between database overlap and inclusion in the active set.
We identify 158 and 166 active reactions in E. coli and S. cerevisiae, respectively, accounting for more than half of all redundant reactions or 75 80% of nonefficient redundant reactions (fig. 2).
Among the pathways with highest relative number of active reactions in macrophages were several fairly ubiquitously active pathways such as hyaluronan metabolism, chondroitin sulfate degradation, and N-glycan degradation (Figs. 1 and 3).
For a more precise determination of active reactions in each growth condition, we performed flux variability analysis (FVA) [ 44] to determine possible ranges of fluxes of all reactions that still satisfy the same optimal growth.
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We studied the femur-tibia joint control system of the insect leg, and its switch between resistance reflex in posture control and "active reaction" in walking.
Most reactions participate in less than 10% of ExPas, especially in TRN, sTC and sTL, but a few active reactions participate in many ExPas.
From the sampling analysis, a considerably larger number of significantly perturbed reactions are predicted in the anaerobic case (505 reactions, or 70.7% of active reactions) than in aerobic (394 reactions, or 49.8% of active reactions).
A full listing of all differentially active reactions found in the expression data and the final context-specific models are provided in the Supplementary Material for further research (see Additional File 6).
Repeating this calculation for the internal stoichiometry matrix of each of the six subnets gives (42, 17, 5, 47, 2, 2) active reactions respectively in each subnet.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com