Transcription analyses suggest that the genes involved in acetoin, 1,3-propanediol and adenosyl-cobalamin biosynthesis were differentially regulated upon the glp system activation, demonstrating the cross-talk between silent and active pathways.
This TF activity array provides a large-scale view of active pathways within a system at multiple time points.
In between these time points, metabolic activity was constant; expression of the genes in the active pathways was neither enhanced nor diminished, within the experimental error limits.
This study focused on analyzing active pathways and the metabolic flux distribution in human neuronal AGE1.HN cells that is a desirable basis for a rational design and optimization of producing cell lines and production processes for biopharmaceuticals.
Constraint-based modeling results obtained in this work revealed that the metabolic impact due to heterologous protein production is widely distributed in the genome of S. lividans, causing both slow substrate assimilation and a shift in active pathways.
By reengineering the obsolete streets, we can create robust and ecologically active pathways.
Three labeling data were used to trace the carbon metabolic route and to identify active pathways.
Vert and Kanehisa [14] extracted active pathways from gene expression data by using the metabolic network from the KEGG database as prior knowledge.
We have established inducible UMP/CMP kinase cell lines as cell models in an attempt to understand the active pathways of nucleotide metabolism.
Structural plasticity, resulting in changes in the dynamic structure of substrate fluxes that present on-off changing states, so previously inactive catalytic processes are turned on while previously active pathways are turning off and viceversa.
The investigation of active pathways and cellular responses performed within an environment where cells receive signals from the extracellular matrix, neighboring cells, and soluble factors within the local milieu would be an effective means of elucidating regulatory mechanisms.
