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Hence, B. stratosphericus lipase has preferred the high temperature of 50 °C for maximal activity although the lipase was active over a broad range of temperature between 30 and 80 °C.
The activity of the recombinant Xyn2 was highest at 60°C and it was active over a broad range of pH (3.0 8.0) with maximal activity at pH 6.0.
However, many of these enzymes were active over a broad pH range, and all but J16 retained ≥50% activity at pH 7.0.
As shown in Figure 5C, FSAΔSK was active over a broad temperature range (20°C to 100°C) and had an optimum activity at 55°C.
The enzyme was active over a broad temperature range (40 60 ± 1 °C) and pH (7.0 9.0) with maximal activity at 60 ± 1 °C and pH 8.0.
CauloGA was optimally active at pH 5 to 6 and remained active over a broad pH range, between pH 4.5 and pH 7.0, at which the enzyme demonstrated more than half of its maximal activity.
Similar(47)
However, the N321H mutated enzyme was active over a broader pH range compared to the wild type, with no much change in the specific activity.
Both enzymes are active and stabile over a broad pH range and also their activity is completely inhibited by PMSF suggesting that they both are serine proteases.
Ideally, alkaline proteases used in detergent formulation should demonstrate high level of activity and stability over a broad range of pH and temperature, possess broad substrate specificity and be active in the presence of detergent ingredients, such as surfactants, bleaching agents, fabric softeners, and other formulation aids [7].
Mr. Pincus was active in a broad range of philanthropies.
As befits a sporting dog, it is muscular and active, with a broad skull and hanging ears.
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