Knockdown of any one of these three proteins alone does not induce activation of the Xi-linked GFP reporter, but rates of activation increase when knockdown is sensitized by a DNA demethylating agent and a histone deacetylase inhibitor [ 21, 22].
Bcl10 filament formation is a highly cooperative process whose threshold is sensitized by oligomerized CARMA1 upon receptor activation.
Taken together, these data suggest that TRPA1 activity could be sensitized by the activation of numerous GPCRs or receptor tyrosine kinases (RTKs) via the cAMP/PKA and PLC/PKC pathways following Ca2+ elevation (Taylor-Clark et al., 2008; Andersson et al., 2008; Taylor-Clark et al., 2009).
Once nociceptors are sensitized, the threshold for activation is decreased, discharge rate with activation is increased, and rate of basal (spontaneous) discharge is increased, resulting in easier response of nociceptors to incoming stimuli [ 19].
Based on the strong correlation of p-p38 and p-JNK metrics with cell death in the PLSR model and the differential cell death response to p-p38 inhibition, we hypothesized that infected cells may be sensitized to targeted activation of these pathways.
For classical pathway complement activation, sheep red blood cells (SRBCs) were sensitized with rabbit anti-SRBC (1 : 400) (antibody ab50676, Abcam, Cambridge, MA, USA).
The consequences of RAS activation on CQ sensitivity also varied depending on the cell type, with some cell lines being sensitized to CQ by oncogenic RAS and others becoming more resistant.
Moreover, evidence suggests that mechanoreceptors are sensitized by metabolites accumulation, thereby enhancing sympathetic response arising from their activation and making it difficult to isolate pure mechanostimulation from metabostimulation [ 154].
All these genetic data are consistent with MblC overexpressing eye discs being sensitized to enter apoptosis, although we did not detect increase in caspase-3 activation in third instar eye imaginal disc overexpressing MblC (not shown).
Our finding suggests that the LPL-T of IBD patients are sensitized to APN in the absence of a stimulus that leads to STAT3 activation.
