The assay using purified reagents and verifying the cofactor activity of the recombinant factor V molecules for prothrombin activation was conducted under conditions where all factor Xa was saturated with factor Va, as described by measuring α-thrombin formation by the change in the absorbance of a chromogenic substrate at 405 nm (Spectrozyme-TH, 0.4 mM) (16).
Mechanical activation was conducted by pestling the particles in a ceramic mortar.
The activation was conducted at 1,373 K in presence of Ar and reactivation was conducted in presence of (steam + Ar) flow at 573 K.
Then, hydrogen activation was conducted at a pressure of 1.2 MPa and a temperature of about 140 °C to remove other surface-adsorbed gases.
After the chamber was pumped down to 3.0 × 10−3 Pa, the pre-treatment of template fibers for surface activation was conducted by the H2 gas and Ar gas injected into the chamber for 10 min. The applied radio frequency power was 60 W.
The rotating disk electrode (RDE) measurement of the catalysts after activation was conducted by scanning the electrode potential from 0.8 to −0.2 V (vs. SCE) at a rate of 5 mV · s−1 and with an electrode rotating rate of 900 rpm in argon and oxygen-saturated 0.5 M H2SO4 solution, respectively.
Characterization of the TNFα expression and the NF-κB activation was conducted by immunohistochemistry in paraffin-embedded lung tissues (4 µm) isolated from silicosis patients at time of lung transplantation.
Studies of cyanide activation were conducted with the ACCO His-Tag fusion protein after cutting the N-terminal His-Tag leader sequence with thrombin.
An initial regression of age and average magnitude of activation was conducted to estimate the effects of these potential confounding variables (Table 3, Model 1).
Slope of activation was entered as the dependent variable, and an initial regression of age and average magnitude of activation was conducted to estimate the effects of these potential confounding variables (Table 2, Model 1).
The conditions for the PCR amplification were as follows: polymerase activation was conducted at 95°C for 30 s; followed by 40 cycles at 95°C for 5 s, 60°C for 31 s.
