NKG2D expression levels on lymphocytes before and after activation were analyzed by fluorescence-activated cell sorting.
Caspase-3 activation was analyzed by immunofluorescence analysis using a specific anti-active caspase-3 antibody.
The gas products during activation were analyzed online to reveal the activation mechanism.
Polymorphonuclear neutrophil activation was analyzed by measuring cell surface and soluble cell adhesion molecule expressions.
Whole blood and peripheral blood mononuclear cells (PBMCs) were stimulated with poliovirus vaccine, and memory T cell activation was analyzed by flow cytometry and lymphoproliferation, respectively.
Coagulation cascade activation, early platelet adhesion and activation were analyzed after 2 h of exposure of blood to the biomaterials.
MAPK and PI3K signaling pathway have been reported to be involved in macrophage activation22, 23, the effects of andrographolide on the signaling pathway activation were analyzed by detecting phosphorylation level of ERK 1/2 (MAPK pathway) and AKT (PI3K pathway).
The phase and molecular structures of hwangtoh before and after alkali activation were analyzed by X-ray diffraction (XRD) and solid-state nuclear magnetic resonance (NMR).
To gain insight into its action mechanism, methanol OH bond activation was analyzed over MgAl mixed oxides using in-situ DRIFT spectroscopy to follow adsorbed species.
The graphitic and pore structures of the coke after the electrochemical activation were analyzed by XRD to confirm retention of the graphene structure.
The role of each component of the catalyst during the activation was analyzed by studying the behavior towards SO2 adsorption of four materials, which contained: diatomaceous earth, diatomaceous earth + V, diatomaceous earth + K, and diatomaceous earth + V + K.
