The functional activity was confirmed by plasminogen activation assay and in vitro clot lysis assay.
In order to determine Ras activity, we used a Ras activation assay kit (Millipore).
Ras activity was determined using a Ras activation assay kit (Millipore, Watford, Hertfordshire, UK).
Ras activity was assessed using the Ras activation assay kit from Upstate Biotechnology (Temecula, CA, USA).
RAS activity was assessed by employing the RAS activation assay kit (Upstate Biotechnology).
The activity of Ras was analyzed with Ras Activation Assay Kit from Millipore (CA, USA) according to the manufacturers recommendations.
The results from the T-cell activation assay revealed that binding acyclovir did not activate T-cells.
The level of active Raf-bound Ras was assessed utilizing a Ras activation assay kit (Upstate, Lake Placid, NY).
Transcriptional activation assay in yeast indicated that ONAC045 functioned as a transcriptional activator.
The RhoA activation assay was used to assess RRIG1-induced inhibition of RhoA activity.
Their activities were evaluated by competitive binding assay with recombinant human ERα, transcriptional activation assay and cell proliferation assay.
