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We then evaluated PH-mCh translocation as above to determine the effector activation abilities of M3-αq-CFP in the presence of YFP-γ2 or γ9 in CHO cells.
To obtain a complete phenotypic rescue, Oa1−/− melanocytes were stably transduced with retroviral vectors carrying either the wild-type human OA1 (Lor1SN), or a mutant that carries a deletion of 18 amino acids in the third cytosolic loop (LOA1Δ18SN), and that had been previously shown to lack G-protein activation abilities, despite an apparently normal subcellular distribution (25).
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Under reaction conditions, the active species will be oxidized to ferric oxide, thus O2 activation ability and catalytic activity decrease.
However, when it was extracted with methanol, acetone or hexane, the extracts showed some resistance activation ability, albeit the activity was relatively low.
The characterization results showed that the pretreatment obviously promoted the amount of the active Mn4+ species and oxygen activation ability.
(4) Quantitative characterization of the activator molecule is given using some topological indices, with which the activation ability can be predicted, and screening of the proper activator is made possible.
Different from roles of carbon quantum dots previous reported, the improved photocatalytic activity was mainly attributed to the molecular oxygen activation ability of N-CQDs, which derived from the oxygen defects on the surface of N-CQDs.
For example, Balboa et al. found increased activation ability with more VP16 fusing together.
The keratinocyte activation ability of chemicals was represented by data from KeratinoSens and LuSens assays.
The LLNA assays representing the T-cell activation ability of chemicals were collected from NICEATM LLNA database [32].
Single-conidium and single-chlamydospore isolates of H. phialophoroides were each cultivated in liquid medium and the cultures were tested for resistance activation ability as described above.
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