Nodes belonging to circuits significantly more activated in the first condition appear in blue while nodes significantly more activated in the second condition appear in red.
Next, we explored the possibility that transcription factors activated in the second wave might regulate the expression of genes activated in the third wave.
We conclude that some genes in the third wave are downstream targets of transcription factors that are activated in the second wave.
We show that polyadenylation of maternal mRNAs is required for the establishment of normal levels of zygotic transcription, and for two transcription factors activated at the MBT we identify downstream target genes that are activated in the second wave of zygotic transcription.
Experimental analysis indicates that the polyadenylation of maternal transcripts is necessary for the establishment of proper levels of zygotic transcription at the MBT, and that genes activated in the second wave of expression, including Brachyury and Mixer, contribute to the regulation of genes expressed in the third.
To do so, we chose two genes activated in the second wave the putative targets of which we could infer from a meta-analysis of gene regulatory networks in early Xenopus development (Loose and Patient, 2004), as well as from specific studies of the two genes in question: Mixer (Sinner et al., 2006) and Brachyury (Gentsch et al., 2013).
Nuclei identified as Not Reactivated by protein (ARC+FOS−) from both the A > A and A > C exposures clustered with the late signature, indicating that these neurons activated in the first environment but not the second.
More than 3,600 nonresidential systems were activated in the first half of 2012, bringing the number of individual solar electric systems to 24,000, the report said.
In later stages, the signal transduction pathways activated in the first phase trigger the production of different proteins and compounds that restore or achieve a new state of homeostasis.
Similar to the previous strategy, but methods are enabled in different order, i.e., PTS is activated in the first half of the simulation and MTSC in the second half.
