To study gene function during later stages, morpholinos can be activated conditionally by light-induced uncaging (Shestopalov et al. 2007).
26 As we discussed in the introduction section, several methods that have been developed to discover denovo pathways or pathway segments do not focus on conditionally activated pathway identification, 2– 4 and cannot output differentially activated pathway segments.
In this paper, we propose an efficient method to identify conditionally activated pathway segments starting from the identification of conditionally activated TFs, by incorporating protein-DNA binding data, gene expression data and protein interaction data.
Characterisation of the Wnt antagonists and their response to conditionally activated Wnt signalling in the developing mouse forebrain.
The firing order of actors (schedule) is determined at compile-time, however, the actors are conditionally activated at run-time.
Furthermore, expression of genes critical for β-cell endocrine function e.g. HNF4α and HNF1α was increased in mice when NFATc1 was conditionally activated [37].
Indeed, the Akt cell survival pathway is conditionally activated only upon exposure to cytotoxic insults or in abnormal cells that require exceptional survival techniques, such as cancer cells.
In this study, we made use of a strain of mice containing a mutant K-Ras allele (K-RasG12D) that can be conditionally activated by Cre-recombinase mediated excision of a floxed transcription termination (LSL) cassette [16].
A more dynamic pattern is observed for clusters that are conditionally activated, most often with complex members present at early times and then absent at later times to yield a smaller core complex.
While the genetic approaches used to conditionally activate or delete β-catenin provide insights onto the role of β-catenin in intermediate progenitor development, further studies are required to determine whether β-catenin controls intermediate progenitor development through direct regulation of genes such as Tbr2 or Insm1.
Therefore, conditionally activated Akt directly inhibits expression of Mirk.
