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We have shown that the purified plexin cytoplasmic region displays low RapGAP activity, which can be activated dramatically by fusing it to the coiled-coil dimerization motif of GCN4 (general control non-repressed 4) (Wang et al., 2012).
Both pathways are activated dramatically by ionomycin, which induces an artefactual calcium efflux pathway in the mitochondria, while the increased cytoplasmic calcium generated by the ionophore at the plasma membrane increases calcium entry into the mitochondria through the uniporter.
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Overexpression of ETS-1 increased the activity of EBS-Luc (Fig. 3d); whereas this activity activated by E2 decreased dramatically in the down-regulation of endogenous ETS-1′s (Fig. 3d) protein level via its siRNA in SH-SY5Y cells.
We found that Src was dramatically activated during platelet activation and, because platelets don't have a nucleus, we knew that it couldn't be regulating proliferation.
Along with the ChIP-chip data [ 24], one group recently suggested that CREB1 is able to bind the CRE site (CGTCA ; −921 to −917 ) on the Atf4 promoter and a constitutively active PKA catalytic subunit dramatically activated Atf4 promoter through PKA-CREB1 signaling [ 23].
As expected, the catalytic properties of activated sample improved dramatically as compared to non-activated ones.
As predicted, ERRγ dramatically activated YY1 promoter, which was repressed by expression of SHP (Figure 4a).
Furthermore, a report using chemical inhibitors indicated that ERK pathway via PRKD1 was dramatically activated by Tat in monocytes [36].
FAC also dramatically activated the MAPK and PI3K pathways in HEY cells.
All six ABA-inducible genes were dramatically activated by the ABA treatment in wild-type plants.
Furthermore, we found p38 pathway was dramatically activated by MDK after one hour treatment, while c-jun NH2 terminal kinase pathway was not activated (data not shown).
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