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Endoglucanases action on substrates with different crystallinity; Figure S4.
The several catalytic modules on the same polypeptide chain are assembled such that their enforced proximity account for an enhanced concerted action on substrates (Moraïs et al. 2012).
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Despite recent controversy on the role of sirtuins in lifespan extension, their actions on substrates known to play important roles in fundamental cellular processes related to the health status of the organisms, such as FOXO transcription factors, α-tubulin, PEPCK1 and NF-κB, indicate that they must be central players in the biology of cells and organisms.
We have done extensive studies to rule out the possibility that the action on the substrates was due to a cytosolic contamination or a mitochondrial leak.
This parameter changes in response to the physical and chemical conditions that allow the development of microbial metabolic processes and its action on organic substrates.
Microbiological activity is an indicator that the soil dynamic reflects the physical and chemical conditions that allow for the development of the metabolic processes of microorganisms and their action on organic substrates [43].
Previous studies indicate that GH51 Abfs have various modes of action on different substrates.
These engineered MCs have additional features that will allow exploration of other interesting aspects of topoisomerase action on supercoiled substrates.
The presence of several catalytic modules in the same polypeptide chain would seem to indicate that their enforced proximity would account for an enhanced concerted action on cellulosic substrates.
An identifier is only established if the peptidase in question can be shown to differ from an existing peptidase because there is a significant difference in the action on one or more substrates or in the interaction with one or more inhibitors, or, more likely, because the sequence is too divergent to be included within an existing MEROPS identifier.
However, it is not well understood how pH influences the enzyme conformation required for catalytic action on the substrate.
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