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Three transcriptional regulatory proteins, DnaA, GcrA and CtrA, acting sequentially regulate more than 200 cell cycle-regulated genes [16].
The serially expressed AP2 genes provide attractive candidates for transcriptional specificity determinants acting sequentially during Toxoplasma replication, although to advance this model the AP2 mRNA profiles across the parasite cell cycle must translate into ordered protein expression and translocation of AP2 factors to the nuclear transcriptional machinery.
The biosynthesis of PGE2 requires two enzymes acting sequentially.
The biosynthesis of PGE2 from membrane lipids is catalyzed by three groups of enzymes acting sequentially.
It also can be accomplished by a combination of Rev1 and pol ζ acting sequentially.
The levels of bacterial biofilm regulator c-di-GMP are controlled in E. coli by two diguanylate cyclase/phosphodiesterase pairs acting sequentially, YegE/YhjH and YdaM/YciR.
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However, the two Arabidopsis proteins act sequentially, with AtPHT1 9 acting in cell layers closer toward the root surface than AtPHT1 8.
MAPK protein phosphorylation cascades are typically comprised of three sequentially acting protein kinases, a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK), and a MAPK.
A protein-based shell encapsulates a series of sequentially acting enzymes in order to sequester certain sensitive metabolic processes within the cell.
Our kinetic model provides a theoretical basis for engineering proximity channeling between sequentially acting enzymes in microbial cell factories and enzyme engineering.
Compared with protein enzymes assisted bienzyme aptasensor, this new aptasensor avoided the fussy labeling process and the spatial distribution of each sequentially acting enzyme, which provided ideal candidate for the development of sensitive and simple bioanalytical platform.
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