This indicated that their promoters contain unique cis-acting elements.
In addition, multiple cis-acting elements were identified in pSH4 in this study.
Interestingly, the expression pattern coincides with a number of cis-acting elements presented in pSH4.
Cis-acting elements are essential genomic sequences that control gene expression.
Multiple cis-acting elements were also detected in pSH4 (Table 1).
Elements regulating transcriptional process are composed of both cis-acting and trans-acting elements [50, 51].
The stable cell line can be used to identify the trans-acting elements with siRNA.
Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (DRE).
When this method was applied to O. sativa subjected to zinc deficiency and Arabidopsis thaliana subjected to salt stress, several novel candidate cis-acting elements that overlap with known cis-acting elements, such as ZDRE, ABRE, and DRE, were identified.
Many cis-acting elements essential for the regulation of gene expression have been identified, mostly upstream of transcribed sequences.
