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We knocked down these effectors in RhoGEF2 + Raf GOF (or Ras ACT) clones, using the previously validated RNAi transgenes rok RNAi (Xu et al., 2008), dia RNAi (Widmann and Dahmann, 2009) and PKN RNAi (Warner and Longmore, 2009).
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As most of the tested mutant combinations affected viability, we could disrupt ecdysone signalling during development only via induction of single cell clones using the act< CD2< Gal4, hsFlp system and via EcR overexpression.
Plasmids were purified from positive clones using a QIAprep spin miniprep kit (Qiagen, CA), and cloned in pTZ57RT cloning vector using InsTAclone PCR cloning kit (Fermentas, UK).
To determine whether mer acts independently of the core components of the pathway, we reexamined the role of mer in oogenesis by generating follicle-cell clones using a null allele, mer4 [56].
Deposit your clones using the "place" action.
The clones used were fifteen clones.
Both Harvard and California, San Francisco, have announced plans to do therapeutic cloning using private money.
Dolly was the first mammal to be cloned using DNA taken from an adult cell.
PCR products were cloned using TOPO TA Cloning Kit (Invitrogen).
In 1996, Dolly the sheep became the first animal to successfully be cloned using this method.
Fragments were cloned using InsTAclone PCR Cloning Kit (Fermentas, Lithuania).
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