In the second component we apply several pre-processing steps like face alignment and lighting normalization to ensure comparability of the facial images across the entire database and improve the systems robustness against lighting changes.
We analyzed the behavioral domain and paradigm class metadata of BrainMap experiments associated with seed voxels to determine the frequency of domain 'hits' relative to its likelihood across the entire database.
For the purposes of this analysis, we defined genes that were highly expressed based on their normalized expression being at least 4 times higher in an individual immune tissue relative to their median signal across the entire database.
For initial analysis, the raw signal intensity for each probe set ID of interest for each patient was normalized to the median value of the probe set ID across the entire database and subsequently log2-transformed.
Accordingly, in Stage 1 the concept map was used to display all found concepts and their semantic proximity to one another across the entire database of 789,712 abstracts.
To identify and quantify sequence reads per mutant in a given sample, raw sequence data were aligned against the database using Bowtie 0.12.7 (Langmead et al. 2009); only a single mismatch within the first 42 bp were tolerated, and only unique matches across the entire database were retained.
