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PS-CFP2 provided bright violet-blue fluorescence that could be used together with eGFP and mRFP for live cell three-color imaging without signal bleed across filter sets (Semrock DAPI 5060b, GFP3035b, TXRed 40404b), even at long exposure times (>1 second/channel) with very high over-expression of fusion proteins (See Fig. 1a-d).
It shows the potential of extending the model to describe pressure drop across aerosol-loaded filter of increased thickness with non-uniform particle deposition across filter depth being taken into account.
EVs have negatively charged phospholipid membranes and therefore move across filter membranes.
Then, we present two new tap selection schemes which apply the M-Max criterion independently in each sub-filter across filter length only.
Electrophoresis-driven filtration exploits the negatively-charged phospholipid membranes of EVs to move them across filter membranes, whereas proteins and other molecules have different charges due to side chains of amino acids.
In order to avoid this problem, two new schemes have been presented which perform tap selection by applying the M-Max criterion only across filter length (and thereby exploit temporal sparsity for updating each sub-filter) and do not completely ignore the sub-filters with the smallest magnitude tap-inputs.
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Bidirectional transport of IgE was measured across filter-grown cells, either normal cells or those transfected with antisense CD23 oligonucleotides, or in the presence of blocking antibody.
IgE was transported in both the basal-to-apical direction and the apical-to-basal direction across filter-grown epithelial cells, a process that was inhibited by transfection of cells with CD23 antisense oligonucleotides or pretreatment with nonspecific IgE or anti-CD23 antibody.
(In experiments A-Greedy usually converges to the optimal ordering). One very important feature of A-Greedy is that it monitors and responds to selectivities that are correlated across filters (i.e., that are nonindependent), which provides the strong quality guarantee but incurs run-time overhead.
This analysis identified a small number of genes that appear to be highly variable across filters.
Variability across filters was also evaluated by computing the average hybridisation signal for each gene across three replicates, and then calculating the standard error of the means from each filter.
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