At this point we performed the quantitative analysis and in general we observed large intra-assay variability for most genes across different samples, both for infected (Additional file 4) and for control animals (Additional file 5).
They might show expression divergence as well as individual difference across different samples.
Furthermore, it is not rational to compare R across different samples, which given clinical populations, are likely to differ significantly in the variance of the independent and dependent variables.
The expected coefficients are remarkably stable across different samples.
Fig. 2 Scatter plots of gene expression levels across different samples.
The number of common components across different samples was selected according to the retention times of the common peaks.
The estimates are robust across different samples, specifications and estimation methods and provide evidence of a previously undocumented indirect effect of civil wars on the well-being of children and subsequent economic growth in refugee-hosting communities.
Selecting the IRT model, which is the closest fit to the assessment test data is essential to obtain question's difficulty and discrimination values which are invariant across different samples of students (Hambleton and Swaminathan 1985; Gler et al. 2014).
To ensure fluorescent intensities are comparable across different samples, a constant exposure time (300 mSec) was used.
By analyzing GFP-positive and GFP-negative cells from the same sample in parallel, we were able to perform comparative analysis both within and across different samples.
The raw data was obtained and normalized log2 ratio values were used to identify the differential gene expression levels across different samples.
