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If a component contains a super majority (≥70%) of all heavy (non-hydrogen) atoms across all unique components of a mixture and if that component has at least one carbon atom, it is designated as the parent component.
The NimbleGen Tiling ChIP Service (design ID 19441, design name 090109_Tbru_LM_CHIP) was used: 385,816 probes were tiled across all unique regions of the T. brucei genome at an average spacing of 61 bp.
The average coverage across all unique repeat motifs, calculated from coverage across 40 bp windows, was 19.93 (SD = 33.96) with a few repeat motifs reaching as great as 200× coverage.
For each permutation, the minimum p p (min p p) across all unique strain segregation patterns was captured and used to generate a null distribution of genome-wide minimum p values.
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To correct these errors, for each possible base at each position in the locus, the mean quality score was calculated across all uniques.
Note that it is important that all reads have unique identifiers across all the datasets combined.
There were a total of 105 unique pathways across all the four populations: forty-nine pathwere were common to all four countries, thirty-five were common to three countries, seven were common to two countries, and five were unique to one country.
Briefly, assembled isotigs and singletons were aligned to protein sequences from the Ref-Seq databases [ 41, 42] for zebra finch, chicken, mouse, and human using tBlastX, resulting in 6,269 isotigs (from 3,827 isogroups) and 3,984 singletons being assigned across all 234 unique pathways available for annotation (should refer to Additional file 2).
The numerator was the sum of all unique values presented across the three formats.
Effective branding is all about making a product or service desirable by ensuring it comes across as unique, relevant and appealing to consumers.
Top tip The section along the Milwaukee river follows the original 34 miles first mapped out for the trail across a unique post-glacial landscape renowned among geologists.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com