Three independent events of spacer acquisition from the same source were identified, resulting in only slightly different spacer composition in different CRISPR alleles (Table 2; Technical Appendix 2 Table 1).
(Some post-Sweeney acquisitions dating from the same period are also included).
Each acquisition sputum sample, from which a new strain of M. catarrhalis was first isolated from a patient, was paired with a pre-acquisition sample from the same patient.
Data were processed using DataAnalysis 4.0 (Bruker Daltonics, Billerica, MA), which allowed averaging of several adjacent ECD scans (typically 3 5 during HDX data acquisition) resulting from the same precursor ion.
This incoherence can be reduced if the waveform excitations and its acquisitions are clocked from the same source enabling synchronous digital averaging (SDA).
Given the high turn-over of genes in DNA phages (most POGs are restricted to few phages), isn't it more plausible to interpret a conflict as resulting from several independent acquisitions of the gene (from the same host, or related hosts) in distinct phage lineages?
From an acquisition direction perspective, they might differ: acquisitions on the same level of the value chain as the acquirer would be rated as horizontal acquisitions, whereas acquisitions on different levels of the value chain e.g. an acquirer's supplier would be vertical.
In this study, our aim was to develop a novel methodology that permits the repeated acquisition of Raman spectra from the same cell cultures without prejudicing culture sterility, including investigating whether or not the Raman acquisition process might adversely affect the cells' behaviour.
The procedure is an alternative to the tilt-series tomography technique for a number of nanocrystalline systems, since its application does not require the acquisition of multiple images from the same nanocrystal along different zone axes.
However, the requirement of model samples and/or the acquisition of multiple images from the same nanocrystal along different zone axes may restrict the practical application of these methodologies, especially for those systems that cannot withstand a high electron dose.
