Single dye labeling of samples, hybridization procedures, data acquisition, background correction and normalisation were performed at the PartnerChip facilities following the standard protocol defined by NimbleGen [ 103, 104].
Single dye labeling of samples, hybridization procedures, data acquisition, background correction and normalization were performed at the NimbleGen facility (NimbleGen Systems, Reykjavik, Iceland) following their standard protocol.
34 Motion and distortion corrected images were normalized to the ASL reference scan signal (the first volume of the ASL acquisitions), without background suppression, to account for B1-field variations.
The first acquisition started in November, 2011, when Coal 1−2 of 12,407 working face hadn't been mined and the coal seam was in original state, and the acquisition was background survey for strata; the second acquisition was carried out 4 months later, when nearly half of Coal 1−2 of 12,407 working face had been mined, and half area of the working face was goaf and the other maintained unmined.
As shown in Fig. 2, spatial-temporal segmentation method includes image acquisition system, background updating, change detection, object positioning, and object tracking.
Color acquisition and background fluorescence were optimized by using the DPController and DPManager software (Olympus).
Using time-gated acquisition the background signal can be efficiently suppressed, enabling more sensitive detection of proteins using lanthanide chelate tags than is possible with chromophore-based or conventional fluorophore-based labels.
Pseudocontinuous ASL perfusion images were collected using 3D fast spin-echo acquisition with background suppression, with a labeling duration of 1500 ms and post-labeling delay of 1500 ms, as suggested previously [ 28].
The source was placed at the center of the camera field of view, and a whole-body acquisition of the vial and a background acquisition were obtained before each patient image acquisition on the day (D0) of the radiopharmaceutical injection and 3 (D3) and 6 days (D6) post-injection using the same acquisition parameters as for the whole-body patient image.
The non-interferometric terms in the signal (I r + I s) were eliminated using subtraction of separate background acquisitions, and the source's spectral dependence was removed by normalizing by the reference field.
