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The LC-MS/MS system was controlled by the Masshunter workstation software (LC/MS data acquisition, version B.03.01).
Gold Acquisition (version 4.00, DSI) was used for the recording.
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Spatial working and reference memory were assessed in two aversively motivated learning and memory tasks: the standard and the "repeated acquisition" versions of the Morris water maze escape task, respectively.
TestFlight Groups replaces legacy distribution lists, which were a part of the pre-acquisition version of TestFlight, and also adds a feature that has been available to Android developers using the built-in Google Play beta testing tools.
After data acquisition, Octopus (version 8.2) and VGStudio Max (version 1.2) were used to perform cross-sectional and three-dimensional reconstructions of the sample, respectively.
Raw data were obtained using Illumina BeadScan image data acquisition software (version 2.3.0.13) and analysed using Illumina BeadStudio software (version 1.5.0.34).
Cells were then incubated at 37 °C with 20 μg/ml RNase A in 1 × PBS for 30 min, stained with 50 μg/ml PI for 30 min, and analyzed using an Epics XL cytometer (Beckman Coulter, Miami, FL, USA), EXPO32 acquisition software (version 12, Verity Software House Inc., Topsham, ME, USA), and WinList analysis software (version 7, Verity Software House Inc).
μCT acquisitions were performed using a high energy spiral scan (Skyscan, model 1173, Kontich, Belgium), acquisition software version 1.6/Build 7, at 6.34 μm intervals and submitted to further crosssection image reconstruction (InstaRecon CBR Server-Premium-12-8K/NRecon, version 1.6.5.8) and imaging quantification (CTAn, version 1.12.0.0).
The images were acquired as static whole-body scans using G8 acquisition software (version 1.2.9.3) and reconstructed with maximum-likelihood expectation maximization (MLEM).
The PET scans were performed 3 h and 5 h after injection of the 44Sc-labeled peptides and 3 h after injection of the 68Ga-labeled peptides, using G8 acquisition software (version 2.0.0.10).
The resulting data were displayed and recorded using a WINDAQ data acquisition system (Version 2.54; DataQ Instruments Inc., Akron, OH, U.S.A .. First, 6 animals were used to determine the effect of i.v. adenosine and caffeine on the middle meningeal artery diameter.
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