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Image acquisition, quantification and data analysis were performed using the Affymetrix GeneChip Operating Software (GCOS) v1.4.0.036.
MAS, version 5.0, [ 10] was used for data acquisition, quantification, and normalization.
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QD-based probes have been used for co-immunoprecipitation and Western blot analysis, allowing for simpler and faster image acquisition and quantification than traditional methods (Fig. 4) [105–108].
Acquisition and quantification of images were performed under "blinded" conditions.
Treatment of membranes, image acquisition and quantification was made as previously described [22], [23].
Hybridisation, image acquisition, signal quantification and recalculation of arbitary units to telomere length in kb was performed as described previously [30].
Using tools recently developed for image acquisition and quantification of fluorescence in different cellular compartments [28], we measured the nuc/cyt ratio of NLS-mCherry under different experimental conditions.
Also in the editorial, after noting how molecular cell biology is emancipating itself from an informal, reductive, hypothesis-driven approach by embracing high-throughput data acquisition, rigorous quantification and mathematical modeling, it is forecast by Kritikou et al. [2] that ultimately a "virtual cell" will be developed.
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