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Images were obtained with a matrix size of 256 × 256 two measurements were acquired: section thickness of 2 mm; field of view of 10 × 10 cm.
DKI data were acquired according to the previously described protocol in [ 18, 19] (SE-EPI-DWI sequence with TR/TE: 3200/90 msec, δ/Δ: 20/48.3 msec; FOV: 240 × 240 mm, matrix: 96 × 96, number of slices: 44, 1 signal average acquired, section thickness/gap: 2.5/0 mm, and b-values: 700, 1000, and 2800 sec/mm in 25, 40, and 75 uniformly distributed directions, resp).
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For the purpose of later validation of a parameter for progression of pathology, to be derived from computerised image analysis, based on the measurements acquired, sections were grouped using histopathological criteria, as follows: N: normal artery: intima only a few cells in width along full circumference.
At the same time the microscope was arranged so that the fluorescence camera was acquiring sectioned images at a plane at the very top of the heart.
Here, acquisition of data was simulated either by evaluating the phantom values sequentially for different y-sections (see Fig. 4(d)) or by acquiring sections in a radial geometry (Fig. 4b) to match our proposed acquisition conditions.
To quickly acquire sectioning microscopic images without destroying the structures of intact tissues, investigators often adopt optical tomographic tools like optical coherence tomography (OCT) or confocal laser scanning microscopy.
To obtain full images of brain sections, multiple grey-scale images were acquired per section using Spot Advanced software (Spot Imaging Solutions) and merged into a full image and inverted into black-on-white images using Photoshop CS4 (Adobe Systems, San Jose, CA).
Indeed, the board found that the FBI can disseminate US person information acquired under section 702 "that reasonably appears to be foreign intelligence information" or even data deemed "necessary to understand foreign intelligence information".
Five images were acquired per section.
Eight images were acquired per section, and integrated immunofluorescence intensity was determined using ImageJ software (Windows version).
80 µm z-stack were acquired (one section every 0.42 µm), and cells were scored with respect to their position in the primitive streak or in the epiblast after 3D reconstruction and optical transverse sections using Imaris software.
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