Natural flow systems evolve by acquiring flow configuration in a definite direction in time: existing configurations are replaced by easier flowing configurations.
The ground-based approach requires set-up of a camera within sight of the master channel to acquire flow dimensions and velocity.
H2O2 was removed and the cells trypsinized and stained with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (Thermo Fisher Scientific) and acquired by flow cytometry using an Attune® acoustic focusing cytometer.
Due to the differential analysis data acquired during flow was noisier than data measured in phases where the flow was stopped.
Samples were acquired by flow cytometry.
All samples were then stained with Streptavidin-PECy5 (BD Bioscience) and acquired by flow cytometry.
For measuring the mitochondrial membrane potential (Δψm), fluorescence was acquired by flow cytometry: green fluorescence at FL1-H channel and red fluorescence at FL2-H channel.
Cells were then stained with anti-CD25-FITC, anti-CD4-APC (Insight Biotechnology) and streptavidin-PE (BD Biosciences), acquired by flow cytometry.
Quantitative PCR data shown in Figure 1 were acquired using flow cytometry sorted K562 cells.
