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Such problems are compounded when flow cytometry is performed on mechanically and enzymatically disaggregated solid tissues or on cultured cells, where subcellular debris, apoptotic or necrotic cells, and highly autofluorescent cells may comprise a substantial proportion of acquired events.
Analysis was performed on 100.000 acquired events for each sample.
Viable lymphocytes were gated by forward and side scatter and the analysis was performed on 100.000 acquired events for each sample.
It is also possible that some of the CN-LOH seen in the tumors might in fact represent IBD and not true tumor acquired events.
The study purpose was to test adult algorithms in a paediatric hospital population and make amendments to increase the accuracy of identification of hospital acquired events.
Fluorescence analysis (Green fluorescence, FL-1 filter) was performed with CellQuest software (FACScan; BD Biosciences) on a total of 100,000 acquired events.
Similar(47)
For each acquired event in the test set, the cluster size asymmetry and the energy asymmetry were evaluated.
This could occur either at mitosis as a tumor acquired event or at meiosis as a germ line event [ 17, 26].
It, furthermore, confirmed the TP53 duplication being a somatically acquired event as it was absent from a skin biopsy obtained at the time of the leukemia diagnosis.
A comparison of the cytogenetic profile of patients with CNV14q12 in a familial cohort would indicate whether this is an acquired event secondary to the primary cytogenetic abnormality, or a primary event indicative of faulty DNA replication pathways.
In some of these studies, higher frequency of CDH1 promoter hypermethylation is reported in relapsed MM and plasma cell leukemia, and it is furthermore shown to be an acquired event through the disease course.
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