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To demonstrate the utility of the IFCSD for fluorescence correlation spectroscopy and quantify its performance, we acquired correlation curves of TAMRA dye dissolved in aqueous buffer.
Each acquired correlation data set was analyzed by software supplied by Carl Zeiss with a fitting program (FCS Access Fit software; EVOTEC BioSystems, Hamburg, Germany), or exported to Igor Pro software (IGOR Pro 5.05a; Wavemetrics, Lake Oswego, OR).
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In them, the noise due to molecular discreteness becomes apparent and acquires correlations to give a departure from the behaviour predicted by macroscopic reaction-rate equations [1], [12], [14] [16].
The metabolic profiles acquired for correlation analysis here were obtained from whole cell lysates of MDA-MB-231 and HCT116 human carcinoma cells.
To reduce stress due to overly prolonged measurement times, only high-resolution μCT data from hindpaws were acquired for correlation analysis of bone surface assessment with F-fluoride PET and CT.
Some of these variables, including the angle of the non-kicking foot, acquired high correlations at time intervals that are useful to goalkeepers.
These two models were utilized to match the equilibrium data and acquire the correlation coefficient (R2) values to assess fitting parameters.
Based on the note that the catalog has to be uniformed in seismic hazard analysis, all magnitude scales should be transformed to a single scale like MS. For this purpose, the initial strive is to acquire a correlation between MS and Mb by considering information that is provided by earthquake catalog of the studied region.
A standard T1– T2 pulse sequence[ 39] was used to acquire 2D correlation data.
Prior to correlation, images acquired with different correlative microscopy methods were first resized to the same scale/pixel size.
To determine the effect to which the proteins were colocalized, two channel confocal images (figure 1a) were acquired and a correlation coefficient was calculated based on the pixel wise correlation of the green and red channels.
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