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In the first level of the design process, the designers should generate a feasible design, then seek to acquire robustness, and finally determine the possibility of independence.
Only under the presence of noise in the developmental process, systems acquire robustness through evolution.
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Herein, we aimed at developing a short-term adaptation strategy and an ALE of yeast in a chemostat to investigate the nature of adaptability of S. cerevisiae to the harsh conditions of low pH and lignocellulosic hydrolysates and the stability of the acquired robustness.
Alternatively, ISO12 may have acquired robustness towards other types of inhibitors, particularly phenolics, during the evolution process.
As long as there are many alternative network structures, each of which can fulfill all required functions, multiple functions may acquire high robustness through gradual Darwinian evolution.
Current evidence of the molecular events affecting quasispecies dynamics suggests that it is unlikely that a viral population can acquire mutational robustness under the increased mutation rates associated with mutagenic treatments.
At present, the biological mechanism for cells to acquire both robustness and adaptivity remains largely mysterious.
The extent, to which this support can be given of course, depends on the quality of data acquired and robustness of modelling implemented for each of the modelling steps.
However the first enzymatic pathway linked to additional external compound (to zeaxanthin in this case) is encountered only by generation seven (Fig. 10a), which means that the linear pathway has to be at least seven reactions long before it acquires dietary robustness (shown in red in Fig. 1a).
This result serves to emphasize the high robustness acquired by strain IBB10B05 during evolutionary engineering, despite the fact that increased resistance to conditions of the hydrolyzate was not selected for.
Taking into consideration the recent view of the mammalian circadian clock as a transcriptional network, 2, 35 through which the oscillator acquires plasticity and robustness, it is reasonable to speculate that the clock network contributes to physiological responses by intersecting with cell-specific transcriptional pathways.
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