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Although there is disagreement regarding the relative significance of these mechanisms [20], [28], there is no doubt that prokaryotes use all of them to acquire new functionality.
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A loftier goal is to design protein sequences that will assume novel structures (Kuhlman et al., 2003) or acquire new functionalities.
In the process called "domestication" a transposase loses its original function and acquires new functionality, creating a novel gene.
In doing so, they acquired new functionalities through a cooperative relationship a process termed endosymbiosis or endocytobiosis (Gray et al. 2001; Martin et al. 2002).
Alternatively, they could have acquired new functions.
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We would argue that this allows one of the copies of a gene to evolve with lesser functional constraint [ 54], which eventually could help it acquiring a new functionality (such as, iap and bro genes).
This analysis and the ancient lineage of snoRNAs which likely arose over 2 3 billion years ago [1] allows the formulation of the hypothesis that when snoRNAs started diversifying by copying themselves to other genomic locations and likely acquiring new targets, a subset progressively lost snoRNA functionality and gained new (and possibly miRNA) capabilities (illustrated in Fig. 1).
In addition to the suppression of p53 activity, the presence of mutations in TP53 may change the protein functionality to a dominant-negative form, sequestering WT p53 and other proteins or acquiring new oncogenic roles [ 26].
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