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From the results of the gate-level simulation, we acquire gate activity for the Synopsys Power compiler.
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The ability to acquire gated images in small rodents which have high heart rates has eliminated the heart motion effect (Fig. 2).
Cells were properly acquired, gated and analyzed using CellQuest Software (BD Bioscience).
A total of 300,000 live events were acquired, gated on small viable lymphocytes, and analyzed with FACSDiva software (Becton Dickinson).
Overlaying simultaneously acquired gating charge and fluorescence traces, along with an estimated measure of membrane potential, makes the slowed response of ArcLight fluorescence especially apparent.
(B ) Fluorescence traces simultaneously acquired with gating currents in A. Colors indicate identical membrane potentials as in A. (C ) Normalized gating-charge-versus-voltage (Q-V) curves (triangles; cyan – R217R, grey – R217Q, purple – R217E) and fluorescence-versus-voltage (F-V) curves (circles; blue – R217R, black – R217Q, red – R217E) for ArcLight.
PET-data was acquired using gating-sequences and analysis of sensor data acquired before and after interruption showed that the sensors were working properly after reapplying their clock signals.
At least 50,000 events were acquired and gating was done on small lymphocytes and CD8+ cells.
For each sample the number of total events acquired and gated for analysis was 5000.
Our method achieves accurate reconstructions without requiring any additional hardware for acquiring a gating signal.
About 30 000 events were acquired and gated using forward scatter and side scatter to exclude cell debris.
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