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The city also agreed to pay for upgrading sewage treatment plants in upstate communities, repair many of the region's septic tanks and acquire buffer land around the reservoirs.
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The acquired buffer is compressed in real time and streamed to network and hard disk for storage.
All images were acquired in buffer solution; a commercial fluid cell was used for imaging in fluids.
For this, the amplitude-frequency plot (i.e. resonance curve) and the phase-frequency plot (i.e. phase curve) were simultaneously acquired in buffer solution far away (~ 100 μm) from the sample surface (Fig. 2A and B).
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The cells were then washed with Washing Buffer, acquired on a FACSCalibur flow cytometer, and the data were analyzed using FlowJo software (Tree Star) using 2% Contour plots to set quadrants.
Cells were washed, resuspended in binding buffer acquired in a flow cytometry, as previously described [ 24].
To confirm this, we superimposed the 2D H N HSQC spectra of the untagged and GB1-tagged N-labeled ASC PYD (acquired under identical buffer conditions).
As found with other Hsp70s, binding of nucleotide to the NBD of HscA was dependent on the presence of Mg2+: the 2D H N TROSY-HSQC spectra of apo and ATP-bound [U-N]HscA386(T212V) were nearly superimposable when they were acquired in TED buffer that lacked Mg2+.
Once Ca 2+ are buffered they acquire the measured diffusivity of the buffers (8.4 × 10 −11 m /s; ).
HC detected NMR datasets were acquired in 100% deuterated buffer.
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