Sentence examples for acids were determined using from inspiring English sources
The quality and the quantity of the isolated nucleic acids were determined using the NanoDrop™ 2000 spectrophotometer (Thermo scientific) and 1% agarose gel electrophoresis (Bio-Rad, South Africa).
The concentrations of organic acids were determined using a high-performance liquid chromatograph system (Shimadzu, Kyoto, Japan) equipped with a refractive index detector (RID-10A, Shimadzu) and an organic acid analysis column (Aminex HPX-87H; Bio-Rad, Tokyo, Japan).
The amounts of the organic acids, including lactic, acetic, propionic, gluconic, succinic, oxalic and citric acids, were determined using ion chromatography ICS-30000, Dionex, USA) as previously described (Hu et al. 2009).
Concentrations of organic acids were determined using a Dionex DX-500 ion chromatograph (Dionex, Sunnyvale, CA, USA) equipped with a GP50 gradient pump, CD25 conductivity detector, AS40 Automated Sampler, an AS11-HC ion-exchange column, and eluted with a mobile phase containing sodium hydroxide using Chromeleon software (Dionex, Sunnyvale, CA, USA).
CMCs of fatty acids were determined using N-phenyl-1-naphthylamine (NPN) from Sigma (St . Louis MO) as a fluorescent probe.
Plasma free fatty acids were determined using an enzymatic method by an automated kit according to the manufacturer's specifications (Wako Pure Chemical Industries Ltd ,Osaka, Japan).
The stereochemistry of the synthesized amino acids was determined using 1H 1H-NOESY of their diastereomeric derivatives.
The serum composition of fatty acids was determined using the method by Lepage and Roy [24].
Specificity of the enzymes towards amino acids was determined using the RP-HPLC-fluorescence method based on l-glutamate (the amino acid product of 2-oxoglutarate transamination) quantification.
The number of similar amino acids was determined using GeneDoc computer program (http://www.psc.edu/biomed/genedoc). Amino acid similarity is calculated based on BLOSUM62 matrix (BLOSUM35 matrix gave very similar results - data not shown).
The concentrations of sebacic acid, decane, decanoic acid, and 10-hydroxydecanoic acid were determined using gas chromatography as explained previously (Mishra et al. 2016).
